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In Stock Series | Alkaline Phosphatase Chromogenic Substrates: Powering Precise Microbial Differentiation in Food Safety Testing
2025/12/25 17:02:57 Browse volume(27)Alkaline Phosphatase Substrates
In food microbiological safety testing, the key is how to quickly and clearly screen potential pathogenic bacteria from complex samples. Alkaline phosphatase (ALP), as a common enzyme in bacteria, its activity detection is a classical method for chromogenic media to achieve rapid sorting and preliminary screening of microbial populations.
Centered on the core requirements of food testing for clarity, stability, and repeatability, GeneSeqTools Bioscience & Technology provides a full spectrum and multi-color range of ALP chromogenic substrates, committed to upgrading this classical tool into a more efficient "Visual Sorting Engine", helping your chromogenic media and detection systems achieve superior performance.
I. Working Principle
All substrates in this series are designed based on the indoxyl phosphate core structure. The chromogenic mechanism is precise and efficient: catalyzed by alkaline phosphatase (ALP), the phosphate group of the substrate molecule is specifically hydrolyzed, releasing the indoxyl intermediate. This intermediate rapidly undergoes oxidative polymerization at the enzyme action site, generating insoluble colored precipitates in situ. By precisely designing the substituents on the indole ring, our company regulates the optical properties of the final precipitate, thereby obtaining chromogenic schemes with distinct contrast such as blue, red, and orange, laying the chemical foundation for multiplex detection and accurate interpretation.
II. Core Advantages: Why Choose GeneSeqTools Bioscience & Technology's Substrates?
✅ Sharp Signal, Clean Background
The chromogenic product is an insoluble precipitate, precisely localized and not prone to diffusion, ensuring sharp colony staining edges, significantly reducing non-specific background interference, and improving the accuracy and credibility of result interpretation.
✅ Engineering Friendly, Facilitating Medium System Design
ALP tolerates culture conditions well and is easily combined with other enzyme reactions for design, suitable for the large-scale development and standardized application of chromogenic media.
✅ Multi-Color Coverage, Enabling Multiplex Detection
Provides a complete color spectrum including blue-violet, magenta, and salmon red, supporting the design of multiplex chromogenic reactions on the same plate, enabling simultaneous preliminary screening and sorting of different microbial populations or metabolic characteristics, greatly improving detection throughput.
✅ Salt Form Options, Adapting to Processes
Two forms are available: disodium salt (water-soluble) and p-toluidinium salt (organic solvent-soluble), which can flexibly match different process chains and stability requirements from laboratory R&D solution preparation to industrial kit production.
III. Product List
1.Magenta-Phos Sodium Salt Monohydrate
Chinese Name: 5-Bromo-6-chloro-3-indolyl phosphate disodium salt
English Name: 5-Bromo-6-chloro-3-indoxyl phosphate, disodium salt monohydrate
Abbreviation: Magenta-Phos Sodium Salt Monohydrate
CAS No.: 404366-59-2
Molecular Formula: C8H4BrClNO4PNa2 · H2O
Molecular Weight: 388.45
Specification: 1 g / 25 g / 100 g (Custom packaging supported)
Form: Lyophilized powder
Catalog No.: G03010006
Stock: In stock
Color Developed: Magenta
Application: Good water solubility, facilitating rapid preparation. Suitable for dual-color chromogenic systems that require simultaneous discrimination of Enterobacteriaceae and other ALP-positive bacteria from other target bacteria in food samples.
2. Magenta-Phos p-Toluidinium
Chinese Name: 5-Bromo-6-chloro-3-indolyl phosphate p-toluidine salt
English Name: 5-Bromo-6-chloro-3-indolyl phosphate p-toluidine salt
Abbreviation: Magenta-Phos p-Toluidinium
CAS No.: 6769-80-8
Molecular Formula: C15H15BrClN2O4P
Molecular Weight: 433.62
Specification: 1 g / 25 g / 100 g (Custom packaging supported)
Form: Lyophilized powder
Catalog No.: G03010005
Stock: In stock
Color Developed: Magenta
Application: Excellent stability, suitable for developing ready-to-use food detection kits or chromogenic plates that require long-term storage of chromogenic stock solutions, ensuring batch-to-batch consistency.
3. X-Phos Disodium Salt
Chinese Name: 5-Bromo-4-chloro-3-indolyl phosphate disodium salt
English Name: 5-Bromo-4-chloro-3-indoxyl phosphate, disodium salt
Abbreviation: X-Phos Disodium Salt
CAS No.: 102185-33-1
Molecular Formula: C8H4BrClNNa2O4P
Molecular Weight: 370.43
Specification: 1 g / 25 g / 100 g (Custom packaging supported)
Form: Lyophilized powder
Catalog No.: G03010002
Stock: In stock
Color Developed: Dark Blue
Application: Classic water-soluble formula, suitable for foodborne pathogen immunoassays or nucleic acid hybridization detection using ALP as a reporter enzyme, achieving low background and high signal-to-noise ratio interpretation.
4. X-Phos p-Toluidinium
Chinese Name: 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
English Name: 5-Bromo-4-chloro-3-indoxyl phosphate, p-toluidine salt
Abbreviation: X-Phos p-Toluidinium
CAS No.: 6578-06-9
Molecular Formula: C15H15BrClN2O4P
Molecular Weight: 433.62
Specification: 1 g / 25 g / 100 g (Custom packaging supported)
Form: Lyophilized powder
Catalog No.: G03010001
Stock: In stock
Color Developed: Dark Blue
Application: Industry gold standard raw material, produces deep purple precipitate when combined with NBT, widely used for in situ identification and membrane detection of specific pathogenic bacteria in food samples.
5. Salmon-Phos p-Toluidinium
Chinese Name: 6-Chloro-3-indolyl phosphate p-toluidine salt
English Name: 6-Chloro-3-indolyl phosphate p-toluidine salt
Abbreviation: Salmon-Phos p-Toluidinium
CAS No.: 159954-33-3
Molecular Formula: C15H16ClN2O4P
Molecular Weight: 354.73
Specification: 1 g / 25 g / 100 g (Custom packaging supported)
Form: Lyophilized powder
Catalog No.: G03010010
Stock: Available to order
Color Developed: Salmon
Application: Provides a unique Salmon red color, offering better background contrast when detecting samples with dark food matrices (such as meat, sauces), reducing the risk of misjudgment.
IV. Color Selection Guide
GeneSeqTools Bioscience & Technology provides three indoxyl phosphate substrates with different hues, all featuring high purity and low background, helping you accurately design chromogenic schemes:
🔴 Magenta-Phos Series (Magenta/Red-Violet)
Chromogenic Effect: Bright Magenta
Core Advantage: Ideal for multiplex detection. Forms a strong contrast with blue, particularly suitable for differentiating ALP-positive indicator microbial populations from other targets on the same detection platform, enabling visual sorting.
Recommended Products:
Magenta-Phos Sodium Salt Monohydrate(CAS: 404366-59-2 , Disodium Salt)
Magenta-Phos p-Toluidinium (CAS: 6769-80-8 , p-Toluidinium Salt)
🔵 X-Phos / BCIP Series (Dark Blue/Purple)
Chromogenic Effect: Dark Blue, turns deep purple when combined with NBT.
Core Advantage: High-sensitivity standard. Sharp signal, low background, a reliable choice for developing detection products targeting specific foodborne pathogens like Salmonella, Vibrio.
Recommended Products:
X-Phos Disodium Salt (CAS: 102185-33-1 , Disodium Salt)
X-Phos p-Toluidinium (CAS: 6578-06-9 , p-Toluidinium Salt)
🟠 Salmon-Phos Series (Salmon Red)
Chromogenic Effect: Unique Salmon Pink (Reddish-Orange).
Core Advantage: Optimized for complex samples. The soft orange-red color is easier to identify against dark food residues or high-background samples, effectively improving interpretation clarity.
Recommended Product: Salmon-Phos p-Toluidinium (CAS: 159954-33-3, p-Toluidinium Salt)
V. Selection Key Points: Disodium Salt vs. p-Toluidinium Salt
Chosen the color? Which salt form to choose? Understand at a glance:
Extended Services
GeneSeqTools Bioscience & Technology Alkaline Phosphatase Chromogenic Substrates – Precise Sorting, Clear at a Glance
For more chromogenic substrates or custom packaging services, please call +86 13302967066!
GeneSeqTools Bioscience & Technology focuses on providing reliable domestic alternative solutions for gene sequencing, molecular diagnostics, and microbiological detection.
For related products such as blood cell dyes and buffer salts, you can view the complete product line and technical specifications on the 【MedSun Biotechnology Website】.
Founded in 2015, it is a high-tech enterprise focusing on microbial testing、 gene sequencing and in vitro molecular diagnostics in the R&D, production, and sales of biochemical reagent raw materials. Committed to providing high-quality biochemical reagent raw materials and professional custom synthesis services, the product portfolio covers more than a dozen categories and nearly a thousand innovative products. Upholding the core philosophy of “customer first, integrity and professionalism”, we serve with dedication to meet customers’ diverse needs in research and testing.
Official WeChat account: Shenzhen GeneSeqTools Bioscience & Technology Co. Ltd.
GeneSeqTools official website: http://www.geneseqtools.com
Hotline: +86 0755-23251735
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