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Hot Product | 3-Indolyl β-D-glucopyranoside (Y-Glc, CAS: 487-60-5): A Chromogenic Substrate for β-Glucosidase Detection
2025/12/9 16:58:37 Browse volume(15)3-Indolyl β-D-glucopyranoside
In fields such as microbial chromogenic media construction, enzyme activity detection, and experimental design for teaching, glycosidase chromogenic substrates are widely adopted due to their "clear reaction, easy interpretation, and broad applicability". As one of the well-established substrates in the β-glucosidase system, 3-Indolyl β-D-glucopyranoside (abbreviation: Y-Glc) continues to garner attention from researchers and media development engineers.
This issue brings you a practical product introduction to help quickly determine if it's suitable for your current experiment or product plan.
I. Product Introduction
Chinese Name: 3-Indolyl β-D-glucopyranoside (Indican)
English Name: 3-Indolyl β-D-glucopyranoside
Abbreviation: Y-Glc
CAS No.: 487-60-5
Molecular Formula: C14H17NO6
Molecular Weight: 295.29
Specification: 1 g / 5 g / 25 g (custom packaging supported)
Appearance: Off-white powder
Product No.: G01040002
3-Indolyl β-D-glucopyranoside is a glycosidic compound, serving as a classic chromogenic substrate for β-Glucosidase (EC 3.2.1.21). Upon enzymatic hydrolysis, it releases 3-indoxyl, which forms a blue indigo precipitate under oxidative conditions, enabling visual detection of enzyme activity. This substrate finds extensive application in microbiology, molecular biology, and enzymology research.
II. Core Advantages
3-Indolyl β-D-glucopyranoside possesses multiple advantages, making it an indispensable substrate reagent in enzymology research and microbial detection:
✅ High Purity: Product purity ≥98% (HPLC). Each batch undergoes strict quality control testing to ensure accurate and reliable experimental data.
✅ High Sensitivity: The blue indigo precipitate generated after β-glucosidase hydrolysis is highly visible, suitable for qualitative and semi-quantitative analysis.
✅ High Specificity: Exhibits high specificity for β-glucosidase, effectively differentiating activities of various glycosidases.
✅ Good Water Solubility: Soluble in water, DMSO, and buffers, compatible with various experimental systems.
✅ Strong Stability: Chemically stable under appropriate storage conditions, suitable for long-term preservation.
✅ Multi-Field Applicability: Suitable for various scenarios including microbial identification, enzyme activity detection, and molecular marker screening.
III. Application Scenarios
1. β-Glucosidase Activity Assay
Used for quantitative or qualitative determination of β-glucosidase activity. Suitable for basic enzymology research, enzyme kinetics analysis, and enzyme inhibitor screening.
2. Microbial Screening and Identification
As a medium additive for screening microbial strains with β-glucosidase activity. Widely used in environmental microbiology, food microbiology, and industrial strain screening.
3. Soil Enzyme Activity Analysis
Assesses β-glucosidase activity in soil, used for soil quality evaluation, organic matter decomposition capacity, and carbon cycle research.
IV. Usage Recommendations
Packaging Specification: 1 g / 5 g / 25 g (custom packaging supported)
Storage Condition: -20°C, store protected from light, sealed, and dry
Experimental Tip: Prepare solutions fresh before use; avoid repeated freeze-thaw cycles.
V. 3-Indolyl β-D-glucopyranoside Frequently Asked Questions (FAQ)
Need reliable and stable β-glucosidase substrate for your research project?
GeneSeqTools Bioscience & Technology has compiled the most common purchasing and usage questions from researchers to help you quickly assess product quality and supply capability.
(1) Product and Technical Specifications
Q1: What is 3-Indolyl β-D-glucopyranoside? What are its main research applications?
A: 3-Indolyl β-D-glucopyranoside (Y-Glc, 487-60-5) is a classic chromogenic substrate for β-glucosidase, widely used for:
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β-glucosidase activity detection
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Microbial strain screening and identification
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Reporter gene detection in molecular biology
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Plant secondary metabolism research
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Soil enzyme activity and ecological assessment
With good water solubility, distinct color development, and high specificity, it is a commonly used substrate in life sciences, environmental sciences, and analytical chemistry.
Q2: What is the chromogenic principle of 3-Indolyl β-D-glucopyranoside?
A: Y-Glc is hydrolyzed by β-glucosidase to release 3-indoxyl. This intermediate product is oxidized in air, and two molecules of indoxyl condense to form a blue indigo precipitate. The blue product enables visual detection of enzyme activity.
Q3: What types of bacteria is Y-Glc suitable for?
A: Y-Glc is used to detect bacterial strains possessing β-glucosidase activity. The specific bacterial groups that can develop color vary depending on the medium formulation, inhibitors, nutrient base, and supplemental components. Common applications include identification systems for some Gram-negative bacteria and some Gram-positive bacteria.
Q4: What is the difference between Y-Glc and X-Glc? How to choose?
A:
Y-Glc is suitable for routine β-glucosidase detection and cost-sensitive applications. X-Glc is suitable for systems requiring clearer color contrast. The specific choice should be determined based on medium formulation and detection requirements.
Q5: What is the purity of Y-Glc provided by GeneSeqTools Bioscience & Technology? Are test reports available?
A: The self-developed and produced 3-Indolyl β-D-glucopyranoside from GeneSeqTools Bioscience & Technology has a purity ≥98% (HPLC). Each batch is certified via HPLC detection and is accompanied by a COA (Certificate of Analysis) and H-NMR spectrum, ensuring reliable, traceable, and reproducible data for research projects.
Q6: What is the solubility of Y-Glc? What solvent is recommended for preparation?
A:
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Appearance: White to off-white crystalline powder;
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Solubility: Soluble in water (approx. 10 mg/mL), DMSO (approx. 50 mg/mL), DMF;
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Preparation Suggestion: It is recommended to prepare a high-concentration stock solution (50-100 mM) using DMSO, then dilute to working concentration with PBS or other buffers before use. Fresh preparation yields the best results;
(2) Procurement, Supply, and Logistics
Q7: Is Y-Glc from GeneSeqTools Bioscience & Technologyavailable from stock? What is the delivery lead time?
A: Yes, our self-developed and produced 3-Indolyl β-D-glucopyranoside is available from stock.
GeneSeqTools Bioscience & Technology is self-developed and self-produced, ensuring stable inventory and enabling:
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Domestic orders shipped within approximately 48 hours
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Rapid supply from mg to g scale
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Support for large-scale, long-term stable supply
This avoids common issues faced by research users such as "stockouts, slow delivery, and significant batch-to-batch variation."
Q8: Is there a Minimum Order Quantity (MOQ) restriction?
A: No minimum order quantity restriction. Flexible options from 100 mg small packages to 5 g, 10 g large packages are available to meet experimental needs of different scales.
Q9: How to obtain the latest quotation? Are there discounts for bulk orders?
A: Inquire by calling +86 13302967066 or emailing sales@geneseqtools.com. Volume orders qualify for tiered discount pricing, especially beneficial for enterprise and platform users.
Extended Services
Choose 3-Indolyl β-D-glucopyranoside from GeneSeqTools Bioscience & Technology to provide reliable assurance for your research.
For high-quality biochemical reagents or custom packaging services, please call +86 13302967066!
GeneSeqTools Bioscience & Technology focuses on providing reliable domestic alternative solutions for gene sequencing, molecular diagnostics, and microbial detection.
For related products such as blood cell dyes and buffer salts, you can view the complete product line and technical specifications on the 【MedSun Biotechnology Website】.
Founded in 2015, it is a high-tech enterprise focusing on microbial testing、 gene sequencing and in vitro molecular diagnostics in the R&D, production, and sales of biochemical reagent raw materials. Committed to providing high-quality biochemical reagent raw materials and professional custom synthesis services, the product portfolio covers more than a dozen categories and nearly a thousand innovative products. Upholding the core philosophy of “customer first, integrity and professionalism”, we serve with dedication to meet customers’ diverse needs in research and testing.
Official WeChat account: Shenzhen GeneSeqTools Bioscience & Technology Co. Ltd.
GeneSeqTools official website: http://www.geneseqtools.com
Hotline: +86 0755-23251735
QQ: 2370561481
Email: sales@geneseqtools.com
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