Chinese Name: 4-Methylumbelliferyl α-L-arabinopyranoside

English Name: 4-Methylumbelliferyl α-L-arabinopyranoside

Abbreviation: 4MU-α-L-Arabinopyranoside

CAS Number: 69414-26-2

Molecular Formula: C15H16O7

Molecular Weight: 308.29

Specifications: 1 g / 5 g / 25 g (Custom packaging supported)

Form: Lyophilized powder

Product Number: G01070026

Stock: In stock

Color: Blue fluorescence

Principle: Releases 4-methylumbelliferone (4-MU) upon enzymatic cleavage, which emits strong blue fluorescence under alkaline conditions (Ex 365 nm / Em 445 nm).

Advantages:

• High Sensitivity: Detection limit down to the nanomolar level.

• Low Sample Volume Friendly: Suitable for scenarios with limited sample volume like body fluids and tissue homogenates.

• High-Throughput Compatible: Adaptable to 96 to 384-well plate formats, supporting large-scale parallel enzyme activity screening.

Applications:

• Detection of low-abundance enzyme activity or samples in complex matrices.

• High-throughput drug screening using enzyme activity as a readout.

• Fluorescence detection channels in rapid microbial identification systems.

Chinese Name: 4-Nitrophenyl α-L-arabinopyranoside

English Name: 4-Nitrophenyl α-L-arabinopyranoside

Abbreviation: PNP-α-L-Arabinopyranoside

CAS Number: 1223-07-0

Molecular Formula: C11H13NO7

Molecular Weight: 271.22

Specifications: 1 g / 5 g / 25 g (Custom packaging supported)

Form: Lyophilized powder

Product Number: G01060058

Stock: In stock

Color: Yellow

Principle: Releases p-nitrophenol (pNP) upon enzymatic cleavage, which appears bright yellow under alkaline conditions and has a strong absorption peak at 405 nm.

Advantages:

• Extremely Simple Operation: Add substrate → React → Stop with base → Read, completed in 3 steps.

• Reliable Quantification: Direct calculation of enzyme activity based on the Beer-Lambert law.

• Instrument Compatibility: Only requires a standard spectrophotometer or microplate reader.

• Established System: pNP substrates are classic standard substrates for glycoside hydrolase activity assays, offering good comparability with literature data.

Applications:

• Defining and measuring enzyme activity units (characterization of parameters like U/mg, kcat, Km).

• Colorimetric wells in biochemical identification panels/strips.

• On-line enzyme activity monitoring during fermentation processes.

• Routine strain screening.

Chinese Name: 5-Bromo-4-chloro-3-indolyl α-L-arabinopyranoside

English Name: 5-Bromo-4-chloro-3-indolyl α-L-arabinopyranoside

Abbreviation: X-α-L-Ara

CAS Number: 1887197-40-1

Molecular Formula: C13H13BrClNO5

Molecular Weight: 378.60

Specifications: 1 g / 5 g / 25 g (Custom packaging supported)

Form: Lyophilized powder

Product Number: G01010020

Stock: Made to order

Color: Blue

Principle: Releases an indoxyl group upon enzymatic cleavage, which spontaneously oxidizes and dimerizes in air to form an insoluble indigo blue precipitate, deposited directly on the enzyme-producing colony.

Advantages:

• Visual Readout: Blue colonies vs. white/colorless colonies, clear at a glance.

• High Spatial Resolution: The precipitate is insoluble and non-diffusible, precisely locating to individual colonies.

• No Instrument Required: The detection method with the lowest hardware requirements.

• Multiplexing Capability: Can be used in combination with other color substrates (e.g., pink Magenta substrates) for simultaneous multi-color discrimination.

Applications:

• Chromogenic media development.

• Direct colony identification in microbiology laboratories.

• As a discriminatory substrate component in selective media.