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Recommended Series | 4-Methylumbelliferone — The Invisible Guardian in Daily Life

2024/12/6 10:54:12 Browse volume(101)
4-甲基伞形酮
4-Methylumbelliferone


Hello everyone! Today, we're diving into the fascinating world of chemistry to unveil the secrets of 4-Methylumbelliferone (4MU) derivatives. You might be unfamiliar with this name, but in fact, 4MU plays an important role in our lives, whether it’s ensuring food safety, monitoring environmental quality, or assisting in medical diagnostics – it’s everywhere.



1 4-Methylumbelliferone in Daily Life
1-1 Guardian of Food Safety

Recently, several outbreaks of E. coli infections in the U.S. due to eating McDonald's hamburgers have once again raised public concerns about food safety. The 4-Methylumbelliferone derivatives we’re introducing today are key reagents for detecting E. coli, widely used in clinical and microbiological research. The detection process for E. coli utilizes the fluorescence properties of 4-Methylumbelliferone. These unique derivatives can act like tiny labels to track microorganisms. Under UV light, these small molecules emit bright blue fluorescence, helping scientists quickly and accurately identify harmful bacteria.




1-2 Pioneer in Environmental Monitoring

4-Methylumbelliferone plays a key role in environmental monitoring. It is widely used to detect microorganisms in water and soil to ensure environmental safety. For example, by detecting specific bacterial groups in water, environmental monitoring personnel can quickly determine if the water quality is contaminated and take timely measures to protect public health.



1-3 A Powerful Tool in Medical Testing

n the field of medical testing, 4-Methylumbelliferone plays an important role. It is used to measure the activity of specific enzymes, aiding doctors in diagnosing diseases. These enzymes play key roles in various physiological and pathological processes, including cell signaling, inflammatory responses, and tumor development. By measuring the activity of these enzymes, doctors can more accurately assess a patient’s health status and develop personalized treatment plans.


2 Detection Principles

2-1 Basic Principles of Enzyme Substrate Method for Coliform Detection

The principle of the enzyme substrate method for detecting coliform bacteria is as follows: After the reagent dissolves in the water sample, total coliform bacteria, fecal coliforms, and Escherichia coli in the sample, when cultured at specific temperatures for a certain period, produce β-galactosidase. This enzyme breaks down the colorless substrate 4-Methylumbelliferone-β-D-galactopyranoside in the selective medium, producing fluorescent 4-Methylumbelliferone (4MU). Escherichia coli also produces β-glucuronidase, which breaks down 4-Methylumbelliferone-β-D-glucuronic acid in the selective medium into 4-MU, which then fluoresces under UV light.


2-2 Fluorescence Principle of 4-Methylumbelliferone

The fluorescence principle of 4-Methylumbelliferone is like a light show in the microscopic world. When specific enzymes act on 4MU derivatives, they cleave the chemical bond between the 4MU and the sugar molecule, releasing free 4MU. The free 4MU, when exposed to ultraviolet light, emits bright blue fluorescence. This property makes it widely applicable across various fields.

3 Recommended Products

In order to better serve researchers, our company has carefully selected a range of high-quality 4-methylumbelliferone derivatives. Below are the recommended products:

1
4-Methylumbelliferyl phosphocholine

CAS Number:97055-84-0

Chinese Name:4-甲基伞形酮磷酸胆碱

Another Name:4MU-Phos Choline

Item Number:G03030006

2
4-Methylumbelliferyl nonanoate

CAS Number:18319-93-2 

Chinese Name:4-甲基伞形酮壬酸酯

Another Name:4MU-Nonanoate

Item Number:G02050006

3
4-Methylumbelliferyl β-D-galactopyranoside

CAS Number:6160-78-7

Chinese Name:4-甲基伞形酮-β-D-吡喃半乳糖苷

Another Name:4MU-Gal

Item Number:G01070001

4
4-Methylumbelliferyl β-D-glucuronide

CAS Number:6160-80-1 

Chinese Name:4-甲基伞型酮-β-D-葡萄糖醛酸苷

Another Name:4MU-GlcA

Item Number:G01070012

5
4-Methylumbelliferyl phosphate, free acid

CAS Number:3368-04-5 

Chinese Name:4-甲基伞形酮磷酸酯

Another Name:4MU-Phos

Item Number:G03010016

6
4-Methylumbelliferyl β-D-glucopyranoside

CAS Number:18997-57-4

Chinese Name:4-甲基伞形酮-β-D-葡萄糖苷

Another Name:4MU-Glc

Item Number:G01070007

7
4-Methylumbelliferyl α-L-arabinofuranoside

CAS Number:77471-44-4 

Chinese Name:4-甲基伞形酮基-α-L-阿拉伯呋喃糖苷

Another Name:4MU-α-L-Arabinofuranoside

Item Number:G01070027

8
6,8-Difluoro-4-Methylumbelliferyl Phosphate

CAS Number:214491-43-7

Chinese Name:6,8-二氟-4-甲基伞形酮磷酸酯

Another Name:DiFMU-Phos

Item Number:G03050001

9
4-Methylumbelliferyl acetate

CAS Number:2747-05-9 

Chinese Name:4-甲基伞形酮乙酯

Another Name:4MU-Acetate

Item Number:G02040006


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