In Sequencing By Synthesis (SBS) technology, the identification of each base relies on a key design: reversible termination and cleavable fluorescent labeling.

Its chemical core lies in — the azido group (-N₃) serving as the linker.

The working principle is as follows:

1. Precise Pairing: Four types of 3ʹ-blocked dNTPs are labeled with fluorophores of different wavelengths. Under the action of DNA polymerase, these fluorescently labeled dNTPs precisely pair with the bases on the sample DNA single strand, extending the complementary strand.

2. Fluorescence Detection: Because the 3ʹ-position of the dNTP is blocked, the DNA chain can only add one base at a time. The sequencer determines which base (A, T, C, G) was incorporated by detecting the fluorescence wavelength in each reaction cycle.

Chemical Process of Fluorescently Labeled dUTP in Sequencing By Synthesis

1. NGS Kit Development: Provides stable and reliable core reaction raw materials for the development of high-throughput sequencing platforms, enhancing sequencing read length and accuracy, and ensuring kit performance.

2. Transcriptome and Single-Cell Sequencing: Provides high-performance raw materials for RNA-Seq and scRNA-Seq, ensuring the accuracy and depth of gene expression profile analysis.

3. Synthetic Biology: Serves as key building blocks for enzymatic DNA synthesis (TdT/EGS), supporting the precise construction of artificial genes and even genomes.

✅ Pure Signal：HPLC purity up to 98%, free from fluorescent impurities, ensuring extremely low background noise and improving sequencing signal-to-noise ratio.

✅ One-Step Cleavage：Staudinger reduction triggers synchronous "fluorophore removal + 3′‑OH reset", reducing residual blocking and residual fluorescence rates.

✅ Comprehensive Channels：Covers 488–650 nm, provides four-color/five-color combinations and filter matching, reducing channel crosstalk.