Chinese Name: 4-Nitrophenylphosphorylcholine

English Name: 4-Nitrophenylphosphorylcholine

Abbreviation: PNP-Phos Choline

CAS Number: 21064-69-7

Molecular Formula: C11H17N2O6P

Molecular Weight: 304.24

Specifications: 100 mg / 500 mg / 1 g (Custom packaging supported)

Purity: ≥98% (HPLC)

Form: Off-white crystalline powder

Product Number: G03030005

Stock: In stock

Color: Yellow

PNP-Phos Choline is a PLC chromogenic substrate. Its principle is to produce a yellow reading at 405 nm after being cleaved by PLC. The substrate structure contains:

• A phosphorylcholine structure recognized by PLC;

• A p-nitrophenyl (pNP) chromophore for signal output;

When PLC catalyzes the hydrolysis of the substrate:

The released pNP forms a yellow anion under alkaline conditions and produces a characteristic absorption peak at 405 nm.

Compared with traditional natural phospholipid systems:

• No complex emulsification required;

• No lipid extraction required;

• More suitable for direct plate reading with a microplate reader;

• Easier to establish standardized PLC activity assay workflows;

1. Quantitative detection of PLC enzyme activity, used for:

• PLC activity determination;

• Enzyme kinetic studies;

• Km/Vmax analysis;

Helping researchers establish a more stable and intuitive PLC colorimetric detection system.

2. PLC inhibitor screening, suitable for:

• Small molecule inhibitor screening;

• Pathway inhibition studies;

• High-throughput drug screening platforms;

The 405 nm standard plate-reading system is more conducive to automated method development and data standardization.

3. Study of bacterial PLC virulence, used for:

• Clostridium perfringens;

• Bacillus cereus;

• Other bacterial PLC-related studies;

Helping establish bacterial virulence enzyme activity analysis models.

4. PLC signaling pathway studies, suitable for:

• PLC-IP3-DAG pathway studies;

• Upstream mechanism studies of Ca²⁺ signaling;

• Membrane signal transduction analysis;

As a classical methodological tool in PLC functional research.

In the raw material evaluation and method development stage, the product design and quality control of PNP-Phos Choline are strictly aligned with industrial application requirements, comprehensively reducing hidden costs from R&D testing to scaled production:

✅ Low Background and Batch Consistency – More Stable Detection Results

Product purity ≥98% (HPLC), helping to reduce blank background fluctuations caused by free pNP and degradation by-products, and minimizing detection bias and method drift caused by batch changes. For long-term PLC enzymology research and automated detection systems, this helps maintain standard curve consistency and data comparability, reducing the risk of revalidation.

✅ Phosphorylcholine Structure Guiding – PLC Detection Closer to Natural Substrate System

The product incorporates a phosphorylcholine head group, making it closer to the natural recognition substrate of PLC, allowing structural differentiation from conventional general phosphatase substrates. Compared to ordinary phosphate ester chromogenic systems, it is more conducive to reducing background interference caused by non-specific enzymatic hydrolysis.

✅ Mature Colorimetric System – Hassle‑free Experimental Implementation

The product is developed based on the classic pNP colorimetric detection route, making it more compatible with existing PLC detection workflows, reducing development pressure from method transfer, automation parameter adjustment, and historical data linkage. It is more suitable for long-term SOP system establishment and automated platform integration.

✅ Domestic In‑stock Supply – Peace of Mind for Long‑term Procurement

GeneSeqTools Bioscience & Technology offers in‑stock supply and flexible custom packaging services, regularly stocking research-grade (hundred‑milligram) to industrial-grade (gram) packs. Domestic in‑stock direct shipment effectively avoids project delays caused by import lead time fluctuations, helping enterprises optimize long‑term raw material procurement costs.