Chinese Name: Suc-Ile-Glu(γ-pip)-Gly-Arg-pNA hydrochloride

English Name: Suc-Ile-Glu(γ-pip)-Gly-Arg-pNA hydrochloride

Abbreviation: S-2732

CAS Number: 1379822-04-4

Molecular Formula: C34H53ClN10O10

Molecular Weight: 797.31

Specifications: 1 g / 5 g / 25 g (Custom packaging supported)

Form: Off-white crystalline powder

Product Number: G04010009

Stock: Made to order

Signal: Yellow

S-2732 is a pNA-based chromogenic substrate, consisting of the synthetic peptide Suc-Ile-Glu(γ-pip)-Gly-Arg coupled with the pNA chromophore via an amide bond. Its core detection logic can be summarized in three stages: "cleavage – release – color development":

1. Enzyme-specific recognition and cleavage

The peptide sequence of S-2732 (Ile-Glu-γ-pip-Gly-Arg) is a specific recognition site for coagulation Factor Xa. FXa is upstream of thrombin in the coagulation cascade and is the core target of heparin's anticoagulant action. When active FXa is present in the system, the enzyme specifically recognizes and cleaves the Arg-pNA bond in the substrate, which is the classical recognition sequence for FXa cleavage.

2. Release of the pNA chromophore

In the intact substrate, pNA is covalently linked to the peptide and does not produce a significant color signal in the un-released state. After cleavage, pNA is released as free p-nitroaniline molecules.

3. Production and quantification of yellow color signal

Free pNA appears yellow in solution, exhibiting a characteristic absorption peak at 405 nm. Absorbance is proportional to residual FXa activity and inversely proportional to heparin activity in the sample.

1. Anti-Xa activity quality control of heparin drugs

Used for the development of anti-Xa chromogenic assay systems and compliance release testing of heparin (including LMWH) as specified by USP/EP pharmacopoeias.

In the anti-Xa detection system, heparin enhances the inhibition of FXa by antithrombin III (ATIII), thereby reducing the residual FXa activity in the system. Applicable for:

• Quality control of heparin drugs

• Potency evaluation of low molecular weight heparin

• In-process testing of anticoagulant drug production

2. Production of anti-Xa detection kits

Used as the core substrate supply for UFH and LMWH anti-Xa detection kits.

S-2732, as an FXa detection substrate, can participate in the construction of:

• Anti-Xa activity detection reagents

• Coagulation analysis supporting reagents

• Automated coagulation detection systems

Helping reagent development companies establish stable FXa activity detection methods.

3. Detection and evaluation of direct oral anticoagulants (DOACs)

Used for the development of detection systems and pharmacodynamic evaluation studies for direct Factor Xa inhibitors such as Rivaroxaban and Apixaban.

By measuring residual FXa activity after drug action, it enables:

• Anticoagulant effect evaluation

• Drug activity studies

• Detection method development

4. FXa enzyme activity analysis and coagulation mechanism research

Used for enzyme activity detection of activated coagulation Factor Xa and coagulation mechanism research, providing a chromogenic detection tool for FXa functional analysis and anticoagulant detection method development.

By detecting the color signal changes produced by S-2732 under FXa action, it supports:

• FXa enzyme activity level analysis

• Coagulation pathway studies

• Optimization of FXa detection methods

Providing chromogenic detection tools for coagulation-related research.

✅ High purity control, ensuring batch-to-batch consistency

• High-purity system: Purity >98% (HPLC), ensuring batch-to-batch stability.

• Controllable background: High purity reduces non-specific background interference and improves signal-to-noise ratio.

• Good water solubility: Easy to prepare, compatible with automated detection platforms.

✅ FXa-specific recognition, providing stable chromogenic detection signals

• Target specificity: Designed specifically for activated coagulation Factor Xa, enabling specific detection of FXa enzyme activity.

• Clear color signal: Releases pNA chromophore, enabling FXa activity analysis through absorbance detection at 405 nm.

• Reliable results: Accurate and reproducible results, meeting the stringent precision requirements of QC and IVD registration.

✅ Pharmacopoeia classic sequence substrate – compliant and well-established, worry-free choice

• USP/EP recognized: Used in heparin and LMWH anti-Xa chromogenic assay systems, compliant with pharmacopoeia requirements.

• Mature application foundation: As a key chromogenic substrate in anti-Xa detection systems, applied in heparin quality control and coagulation diagnostic reagent development.

• Supports standardization system construction: Meeting the quality evaluation, potency determination, and compliance release standards for heparin products, providing solid methodological support.

✅ Reliable supply, cost optimization

• Cost advantage: For the same quality, the cost is significantly lower than imported brands, helping customers reduce costs and improve efficiency.

• Domestic supply: Independently produced by GeneSeqTools, with controllable delivery times and stable supply, reducing procurement cycle risks.

• Multiple specifications available: Available in 1 g / 5 g / 25 g, with custom packaging supported, meeting needs from R&D to full-scale production.