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Alkaline Phosphatase Substrate
AMPPD


In the microscopic battlefield of disease diagnosis, alkaline phosphatase (ALP) acts as a precise "biological messenger," whose activity fluctuations conceal key pathological information such as tumors and infectious diseases. How to capture these subtle changes? As the core raw material for chemiluminescence technology, AMPPD converts the weak signals of ALP into measurable photons through enzyme-substrate specific reactions. This substrate enables accurate and sensitive detection, providing reliable data support for your research.

I. Product Properties

122341-56-4 Structural Formula

Chinese Name: Alkaline Phosphatase Substrate AMPPD, Disodium 3-(2-spiroadamantane)-4-methoxy-4-(3-phosphoryloxy)phenyl-1,2-dioxetane

English Name: 3-(2'-Spiroadamantane)-4-methoxy-4-(3''-phosphoryloxy)phenyl-1,2-dioxetane

CAS Number: 122341-56-4

Molecular Formula: C18H21Na2O7P

Molecular Weight: 426.31

Specifications: 100 mg / 500 mg / 1 g per vial (Custom packaging supported)

Form: Lyophilized powder

Product Number: G05020001

AMPPD is a core substrate specifically designed for alkaline phosphatase (ALP) chemiluminescence detection systems. Through precise molecular design, it achieves highly sensitive photon emission and extremely low background interference, ensuring accuracy and reproducibility of detection results. Its lyophilized form facilitates long-term storage and ready-to-use development, adapting to various scenarios including research experiments and in vitro diagnostic kit production.



II. Application Scenarios

1. Development of assays for tumor markers (e.g., PSA, AFP).

2. Antibody/antigen diagnostic systems for infectious diseases (hepatitis B, HIV).

3. Endocrine function analysis of hormones (HCG, thyroid hormones).



III. Product Advantages

• High sensitivity: AMPPD's unique molecular structure enables highly sensitive photon emission, ensuring high sensitivity.

• Good stability: The lyophilized powder form facilitates transport and storage, effectively maintaining substrate activity.

• High accuracy: Specific enzyme-substrate reaction reduces background interference, ensuring accurate and consistent results.

• Broad applicability: Suitable for various applications including tumor marker detection, infectious disease diagnosis, and hormone analysis.



IV. Detection Principle

The AMPPD molecule features a unique dioxetane structure linking the phenyl ring and adamantane group. Under the catalysis of alkaline phosphatase (ALP), the phosphate group is precisely removed to form a high-energy intermediate. Upon spontaneous decomposition of this intermediate, the dioxetane ring breaks, emitting photons. The luminescence intensity is proportional to the ALP concentration, making it suitable for chemiluminescence immunoassay systems that use ALP as the labeling enzyme.




Extended Services

We also offer a series of related substrates including alkaline phosphatase substrates. For more product information or technical consultation, please call +86-13302967066!

GeneSeqTools Bioscience & Technology is dedicated to providing reliable domestic alternative solutions for gene sequencing, molecular diagnostics, and microbial detection.

For related products such as blood cell dyes and buffer salts, you can view the complete product line and technical specifications on [MedSun Biotechnology Website].


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Shenzhen GeneSeqTools Bioscience & Technology Co. Ltd.

Founded in 2015, it is a high-tech enterprise focusing on microbial testing gene sequencing and in vitro molecular diagnostics in the R&D, production, and sales of biochemical reagent raw materials. Committed to providing high-quality biochemical reagent raw materials and professional custom synthesis services, the product portfolio covers more than a dozen categories and nearly a thousand innovative products. Upholding the core philosophy of “customer first, integrity and professionalism”, we serve with dedication to meet customers’ diverse needs in research and testing.

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